Objective: To test the effect of a human triple-negative breast cancer cell-dendritic cell(DC) fusion vaccine on the proliferation of T lymphocytes in vitro. Methods: DCs were isolated from fresh peripheral blood of healthy donors. The fusion cell vaccine was prepared by fusing the DCs and MDA-MB-231 cells by electrofusion. The morphology of the vaccine was indentified under inverted fluorescence microscope and the phenotypes were analyzed by flow cytometry (FCM). The effect of the vaccine in stimulating the proliferation of autologous T lymphocytes was assessed using CCK-8 kit. Results: The DCs isolated from peripheral blood mononuclear cells highly expressed CD86, CD11c, and HLA-DR on the cell surface. The fusion cells were irregular in shape and coexpressed the phenotypes of both DCs and MDA-MB-231 cells. The fusion cells showed a strong ability to stimulate the proliferation of T lymphocytes in vitro. Conclusion: The triple-negative breast cancer-DC fusion vaccine prepared by electrofusion can strongly stimulate the proliferation of T lymphocytes in vitro.
