Objective: To summary the skill of the establishment of work tract under the guidance of B-ultrasonography for patients with renal caliceal calculi. Methods: 78 cases of renal caliceal calculi were retrospectively analyzed, which had done with percutaneous nephrolithotripsy under the guidance of B-ultrasonography from May 2006 to October 2010. The number and location of percutaneous track, operative time, intraoperative blood loss, and stone-free rate were recorded. Results: All operations were successful without serious complications. Single tract were estabished in 58 cases, dual tracts in 15 cases, and three tracts in 5 cases. In all tract, the tract were mostly through the middle calyx. The stone-free rate was 92.3%. The mean operative time was 45-180 min, and the intraoperative blood loss was 80-350 ml. Conclusions: The efficacy can be improved and the complications should be reduced in percutaneous nephrolithotomy, with the familiar anatomic structure of renal calices and the location of renal calculus under the B-ultrasonography, the operative plan of optimal percutaneous track, and the skill of B-ultrasonography.